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Join us for this free, detailed presentation about liquid chromatography column options.
Stephanie J. Marin is the Clinical and Forensics Global Market Development Manager at Phenomenex. She received her Ph.D. in chemistry from Arizona State University, and has expertise in sample preparation, liquid chromatography and mass spectrometry. She has over 10 years of experience developing and validating clinical methods from her tenure at the ARUP Institute for Clinical and Experimental Pathology. Dr Marin has worked in marketing, product development, and applications development, in addition to customer facing roles in customer and technical support and service and customer education and training. She has also held positions at Hamilton Company, Selerity Technologies and Biotage, and in analytical services for specialty chemicals and polymers at Rohm and Haas (now Dow). She was also a supervisor at an EPA certified laboratory. She is the author of over 30 peer reviewed publications and book chapters and over 100 abstracts presented at national and international meetings.
This webinar will provide insights on column selection for clinical research applications based on analyte properties, stationary phase characteristics, and instrumentation. Reversed phase separation mechanisms and stationary phase modifications, and how they affect retention and selectivity will be presented. How particle size and porosity affect chromatographic efficiency will be explained.
Fully porous vs superficially porous HPLC and UHPLC columns and their use in clinical research will be discussed. LC column selection based on analyte and column properties will be described. Column evaluation for a steroid panel and vitamin method will be presented as examples on how to select the best column.
Who should attend:
Researchers and analysts looking to determine best chromatography approach for method development in clinical analysis
Why attend:
At the end of this webinar, you will be able to:
- Explain how analyte properties and LC separation mechanism affect LC column choice
- Appreciate the differences between fully porous and superficially porous LC columns
- Describe how stationary phase particle size affects chromatography
