HbA1c testing is essential in monitoring and managing diabetes, yet not all results are created equal. In some methods, such as immunoassays and enzymatic assays, additional hemoglobin fractions—like variant hemoglobins and fetal hemoglobin—cannot be distinguished from HbA1c. This interference compromises accuracy, leading to increased rework and misinterpretations that affect patient outcomes.
Ion-exchange high-performance liquid chromatography (HPLC) separates and detects additional hemoglobin fractions, generating a chromatogram that provides more detailed insights into sample composition. This method not only enhances accuracy but also allows healthcare professionals to question unexpected results and conduct further testing when necessary. In diverse patient populations, where hemoglobin variants are more prevalent, this capability is particularly valuable for ensuring reliable HbA1c reporting.
This expert-driven compendium bridges key knowledge gaps in HbA1c testing by exploring the scientific principles behind different testing methods, method-specific advantages and limitations, and best practices for ensuring accuracy and efficiency. With this knowledge, clinical labs can improve testing confidence, enhance result interpretation, and support better patient care.
Download this compendium of resources to explore:
- How testing methodology impacts HbA1c accuracy and reliability
- The effects of hemoglobin variants and fetal hemoglobin on testing results
- Best practices for chromatogram interpretation
- Advancements in HPLC analyzers that are improving operational efficiency
