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Limitations of DNA Biomarkers for Detecting Early-Stage Oropharynx Cancers

TTMV-HPV DNA levels are often indetectable in patients without neck masses

Brigham and Women's Hospital
Published:Nov 01, 2022
|1 min read
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Oropharynx cancers caused by human papillomavirus (HPV) have risen dramatically over the years, superseding tobacco use and heavy drinking as the primary driver of new cases. Fortunately, HPV-positive oropharynx cancers have an improved survival rate compared to other head and neck cancers, allowing for less intensive treatment options, especially if diagnosed at early stages.

One promising biomarker for early diagnosis and predicting reoccurrence, circulating tumor HPV DNA (ctHPV DNA), is found in the blood of almost 90 percent of patients with HPV-positive oropharynx cancer and can be detected using commercially available blood assays measuring HPV DNA from tumor cells (TTMV-HPV DNA). However, in a study of 110 HPV-positive oropharynx cancer patients. Investigators from Brigham and Women's Hospital, a founding member of the Mass General Brigham health care system, found that TTMV-HPV DNA levels are linked to the presence of cancer in the lymph nodes and are often indetectable in patients without neck masses. This has a tremendous impact on how the test is interpreted and applied for early-stage disease and may mean it is not as effective for screening and early diagnosis of this increasingly common disease. 

“ctHPV DNA testing is emerging as a powerful tool in the diagnosis, treatment, and post-treatment surveillance of HPV-positive oropharynx cancer,” said first author Eleni M. Rettig, MD, of Brigham and Women’s Hospital Division of Otolaryngology-Head and Neck Surgery and Dana-Farber Cancer Institute. Rettig is also an affiliated faculty member in the Center for Surgery and Public Health. “It’s increasingly critical to understand both the strengths and limitations of this test.”

- This press release was provided by the Brigham and Women's Hospital