As the cost of sequencing becomes more affordable, the throughput and complexity of NGS assays have risen precipitously. At the same time, the types of samples being used for these assays have expanded as researchers find value in studying low-input, degraded, biological samples, such as cell-free DNA (cfDNA).
In this webinar, Integrated DNA Technologies presents a complete workflow for effective analysis of <1 percent variant allele frequency (VAF) in cfDNA. The webinar will:
- Describe how to increase cfDNA extraction efficiency using magnetic bead-based technology.
- Demonstrate how to maximize conversion, coverage, and complexity from low inputs of cfDNA during library preparation.
- Utilize fixed Unique Molecular Identifiers (UMIs) for bioinformatic error correction to reduce sequencing and PCR errors.
- Discuss how to increase throughput, reduce hands-on time, and minimize errors during complex NGS assays.