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Cushing’s syndrome (CS) is a condition stimulated by a benign tumor on the pituitary gland or a pituitary adenoma. The tumor triggers excessive adrenocorticotropic hormone production, leading to a range of symptoms and complications including weight gain, heart attacks, and stroke. And so, early detection, diagnosis, and treatment are essential to minimize patient risk.  

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The current method of measuring cortisol levels is by chemiluminescence immunoassay (CLIA). While CLIA is the gold standard, it has several drawbacks including a significant false-positive rate. The University of Turin’s division of Endocrinology, Diabetes, and Metabolism has developed a method for the simultaneous quantification of cortisol, cortisone, dexamethasone, and six other exogenous corticosteroids in serum and blood samples.

Read this case study to discover:

  • Details about dexamethasone testing 
  • Why improved means of CS diagnosis are essential 
  • How traditional and new protocols for detecting cortisol, cortisone, dexamethasone, and exogenous corticosteroids differ
  • A detailed protocol, including sample preparation, for accurate detection of CS using LC-MS/MS analysis  in less than 90 minutes from a serum or plasma sample